Highly integrated microfluidic device for cell pairing, fusion and culture

In this study, we proposed a microfluidic device with compact structures integrating multiple modalities for cell capture, pairing, fusion, and culture. The microfluidic device is composed of upper and lower parts. The lower part configured with electrodes and capture wells is used for cell trapping/pairing/fusion, while the upper part configured with corresponding culture wells is used for cell culture. Dielectrophoresis is used to enable accurate cell trapping and pairing in capture wells. Moreover, the paired cells are fused flexibly by either electrical pulses or polyethylene glycol (PEG) buffer. The fused cells are then transferred to culture wells for on-chip culture simply by flipping the device. Using the device and HeLa cells, we demonstrated pairing efficiency of ∼78% and fusion efficiencies of ∼26% for electrical fusion or ∼21% for PEG fusion, and successful cell proliferation and migration after 72 h on-chip culture. We believe that this multifunction-integrated but structure-simplified microfluidic device would largely facilitate cell fusion oriented tasks.