合成生物学
生物
工具箱
计算生物学
大肠杆菌
益生菌
微生物学
基因
计算机科学
遗传学
细菌
程序设计语言
作者
Fang Ba,Yufei Zhang,Xiangyang Ji,Wanqiu Liu,Shengjie Ling,Jian Li
标识
DOI:10.1002/biot.202300327
摘要
Escherichia coli Nissle 1917 (EcN) is a probiotic microbe that has the potential to be developed as a promising chassis for synthetic biology applications. However, the molecular tools and techniques for utilizing EcN remain to be further explored. To address this opportunity, the EcN-based toolbox was systematically expanded, enabling EcN as a powerful platform for more applications. First, two EcN cryptic plasmids and other compatible plasmids were genetically engineered to enrich the manipulable plasmid toolbox for multiple gene coexpression. Next, two EcN-based technologies were developed, including the conjugation strategy for DNA transfer, and quantification of protein expression capability. Finally, the EcN-based applications were further expanded by developing EcN native integrase-mediated genetic engineering and establishing an in vitro cell-free protein synthesis (CFPS) system. Overall, this study expanded the toolbox for manipulating and making full use of EcN as a commonly used probiotic chassis, providing several simplified, dependable, and predictable strategies for researchers working in synthetic biology fields.
科研通智能强力驱动
Strongly Powered by AbleSci AI