生物
桉树
病菌
环介导等温扩增
DNA提取
植物
基因
DNA
聚合酶链反应
微生物学
遗传学
作者
Alishia van Heerden,Nam Q. Pham,Brenda D. Wingfield,Mike J. Wingfield,Jupiter Muro Abad,Álvaro Durán,P. Markus Wilken
出处
期刊:Plant Disease
[American Phytopathological Society]
日期:2024-04-15
卷期号:108 (9): 2731-2739
被引量:3
标识
DOI:10.1094/pdis-01-24-0086-re
摘要
Eucalyptus scab and shoot malformation caused by Elsinoë necatrix is an emerging disease and a serious threat to the global commercial forestry industry. The disease was first discovered in North Sumatra, Indonesia, and now requires a simple and effective method for early pathogen detection. In this study, a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay was developed for E. necatrix. A unique region in a secondary metabolite gene cluster was used as a target for the assay. To test robustness of the assay, LAMP was verified in 15 strains of E. necatrix. A specificity test against 23 closely related Elsinoë species and three fungal species commonly isolated on Eucalyptus showed that the LAMP assay exclusively identified E. necatrix isolates. The assay had a high level of sensitivity, able to detect 0.01 ng (approximately 400 target copies) of pure E. necatrix DNA. Furthermore, using a simple DNA extraction method, it was possible to use this assay to detect E. necatrix in infected Eucalyptus leaves.
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