转染
间充质干细胞
活力测定
干细胞
细胞生物学
细胞疗法
电穿孔
细胞
化学
细胞培养
生物
基因
生物化学
遗传学
作者
Xiufang Liu,Ning Rong,Zhenhua Tian,Joseph Rich,Lili Niu,Peng-Qi Li,Laixin Huang,Yankai Dong,Wei Zhou,Pengfei Zhang,Yizhao Chen,Congzhi Wang,Long Meng,Tony Jun Huang,Hairong Zheng
出处
期刊:Science Advances
[American Association for the Advancement of Science (AAAS)]
日期:2024-04-19
卷期号:10 (16)
标识
DOI:10.1126/sciadv.adk1855
摘要
Transfected stem cells and T cells are promising in personalized cell therapy and immunotherapy against various diseases. However, existing transfection techniques face a fundamental trade-off between transfection efficiency and cell viability; achieving both simultaneously remains a substantial challenge. This study presents an acoustothermal transfection method that leverages acoustic and thermal effects on cells to enhance the permeability of both the cell membrane and nuclear envelope to achieve safe, efficient, and high-throughput transfection of primary T cells and stem cells. With this method, two types of plasmids were simultaneously delivered into the nuclei of mesenchymal stem cells (MSCs) with efficiencies of 89.6 ± 1.2%. CXCR4-transfected MSCs could efficiently target cerebral ischemia sites in vivo and reduce the infarct volume in mice. Our acoustothermal transfection method addresses a key bottleneck in balancing the transfection efficiency and cell viability, which can become a powerful tool in the future for cellular and gene therapies.
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