Electrochemical Chiral Recognition for a Complex System Based on Specific Enzymatic Reactions

Chiral recognition is facing serious challenges in achieving quantitative determination for a definite isomer in a complex sample. Herein, by introducing respectively β-D-glucose oxidase (β-D-GOD) and glucose (Glu) enantiomers (D-, L-Glu) as a model enzyme and analytes as well as carbon nanotubes (CNTs) to immobilize β-D-GOD and accelerate electron transfer processes, an electrochemical chiral sensing platform on the basic of the specific enzymatic reactions was proposed for the first time in chiral recognition. Owing to the high specificity of enzyme, many enzymes can catalyze selectively one enantiomer reaction but have little ability to catalyze the other enantiomers and interferents, resulting that the chiral sensors based on the specific enzymatic reactions have high selectivity and can be used for the direct and selective detection of enantiomer in the complex samples. It's believed the proposed electrochemical chiral recognition strategy based on specific enzymatic reactions will have important application values for direct determination of enantiomer in real samples.