Determination of Tetrandrine and Fangchinoline in Stephania tetradra S. Moore by HPLC

Objective: To establish a method for determination of tetrandrine and fangchinoline fram Stephania tetrandra.S.Moore.Method:The condition for HPLC was C18 column(4.6 mm × 150 mm,5 μm,Agilent),the mobile phase was a mixture of methanol-acetonitrile-water-triethylamine(55∶ 25∶ 20∶ 0.05),the detection wavelength was 282 nm.Result: The standard curve was linear in the range of 0.386-3.86 μg and 0.366-3.66 μg and the correlation coefficient was 0.999 9 and 0.999 9 respectively.The average recovery of total tetrandrine and fangchinoline was 100.1%,RSD 1.79%.Conclusion: The method can be used for the quality control of Stephania tetrandra.S.Moore.