Continuous Analysis of Senile Plaque and Behaviour In APPswe/PSΔE9 Double-transgenic Gene Mouse Model of Alzheimer Disease

Objective To research senile plaque formation and behaviour change process in APPswe/PSΔE9 double-transgenicgene mouse model of Alzheimer disease.Methods Crossbreed the transgenic APPswe gene and transgenic PSΔE9 gene mouse models of Alzheimer disease to produce APPswe/PSΔE9 double-transgenic gene mouse.The genotype of APPswe/PSΔE9 double-transgenic mice were detected by PCR and establish APPswe/PSΔE9 double-transgenic C57BL/6J mouse model of Alzheimer disease.Detection senile plaque formation in 3,4,5,6,9,12 months old mouse brain tissue by immunohistochemistry,modification Bieschowsky silver stain and Thioflavin-S Stain.Behaviour tests of 3,6,9 months old mouse was determined by Morris water maze trials.Results The transgenic APPswe/ PSΔE9 double-transgenic gene C57BL/6J mouse model of Alzheimer disease were established.3 month old mouse can detect behaviour change and 4.5 month old mouse can detect senile plaque formation.senile plaque of 9～12 month old mouse was similar with the plaque of Alzheimer disease patients,spatial memory deficits of 6-9 month old mouse can be observed(P0.05).Conclusions The obvious senile plaque can be detected in brain tissue of 4.5 months old mouse and spatial memory deficits can occur in 3 months old mouse,indicating that the APPswe/PSΔE9 gene transgenic mouse is a useful animal model of AD in Alzheimer disease pathology and medicament research.