The Low Toxic Substitute Reagent for Chloroform in the Extracted Soluble Organic Matter of Sediments

Because chloroform had severe toxicity,other reagents for substituting them were discussed in order to improve the extraction scheme based on conventional extraction analysis.Three single reagents and three mixed reagents were chosen,including chloroform,dichloromethane,petroleum ether,dichloromethane∶acetone(1∶1),dichloromethane∶methanol(7∶1),dichloromethane∶methanol(98∶2) in this experiment.In terms of above mentioned methods,the extracts were separated and the relative contents of saturated hydrocarbon,aromatic hydrocarbon,non-hydrocarbon and asphaltene were analyzed,the results of these methods were compared.Through the analysis,the results indicate that chloroform∶methanol(98∶2) is the best substitute.