The liquid culture conditions of Aspergillus oryzae were studied in this paper.By single factor and orthogonal experimental design,optimum conditions of Aspergillus oryzae were determined.The results showed that under the optimal culture conditions of pH 6.5,culture temperature 37 ℃,maltose 20 g/L,yeast extract 25.7 g/L,the DCW of Aspergillus oryzae over 23.86 g/L was obtained.