Study on Two Patterns Comparison of Gold Immunochromatography Test Strip for Rapid Detection of Lincomycin

The objective of this study was to develop gold immunochromatography test strips to detect lincomycin in milk.Based on two patterns of direct competitive inhibition and indirect competitive inhibition,we optimized concentration of immunogold labeling BSA-LIN(IgG),pH,concentration of coating IgG(BSA-LIN),pretreatment of milk and so on.The results showed that we got two strips that fulfil practical requirement.The LOD of direct and indirect were 30 and 10ppb.Comparing two methods,indirect method was better than direct in sensitivity,stability and testing time.