荧光
水溶液
蒽
化学
溶解度
硼酸
组合化学
色谱法
有机化学
量子力学
物理
作者
Kai Wang,Ruixiao Zhang,Xinmin Yue,Zheng Zhou,Lihuan Bai,Tong Yue,Bei Wang,Dening Gu,Shuo Wang,Yanqi Qiao,Qian Liu,Xue Xue,Yongmei Yin,Rimo Xi,Meng Meng
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2021-03-30
卷期号:6 (4): 1543-1551
被引量:28
标识
DOI:10.1021/acssensors.0c02217
摘要
Reliable and accurate glucose detection in biological samples is of great importance in clinical diagnosis and medical research. Chemical probes are advantageous in simple operation and flexible design, especially for the development of fluorescent probes. Anthracene-based diboronic acid (P-DBA) has shown potential in glucose probing because of its high sensitivity. However, poor solubility limits its applications in aqueous media. In this work, we systemically modify P-DBA by introducing fluoro (F-), chloro (Cl-), methoxyl (MeO-), or cyano (CN-) substituents. Among these probes, the cyano-substituted probe (CN-DBA) displays the highest glucose-binding constant (6489.5 M–1, 33% MeOH). More importantly, it shows good water solubility in the aqueous solution (0.5% MeOH), with ultrasensitive recognition with glucose (LOD = 1.51 μM) and robust sensing from pH 6.0 to 9.0. Based on these features, the CN-DBA is finally applied to detect glucose in cell lysates and plasma, with satisfactory recovery and precision. These results demonstrate that CN-DBA could serve as an accurate, sensitive fluorescent probe for glucose assays in biological samples.
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