The PCR amplified and cloned sequences of rDNA internal transcribed spacer(ITS) region of Pleurotus ferulae,P.nebrodensis and P.eryngii were analyzed.The results were as follows: the relationship of P.ferulae,P.nebrodensis and P.eryngii were close,especially between P.nebrodensis and P.eryngii,but P.ferulae,P.nebrodensis and P.eryngii were three different species.