As industry by-product,Defatted soybean meal prepared for soybean peptide by Bacillus fermenting was one kind of effective utilization of resource.The index the degree of hydrolysis(DH)was used to screen strains,and 4 strains with greater activity of degrading soybean meal were yielded,numbered CHD12、CHD16、CHD21 and CHD27;The index content of soybean peptide was used to evaluate the product of fermentation,and the combination strains(CHD16+CHD27),which were suitable for degradation of soybean meal through solid-state fermentation,were selected from the 4 Bacillus strains;The fermentation process was optimized through two orthogonal tests of(L934) and the results were that the content of soybean peptide in the product was 23.9%,which was 69.5% more than that in the product under the process before optimized(14.1%),and 4.8 times more than that in the medium before fermented(4.14%),and the peptide recovery ratio was 60%.