Effects of shen song yang xin on neural remodeling after myocardial infarction in rabbits

Objective To evaluate the effect of shen song yang xin( SSYX) on neural remodeling after myocardial infarction in rabbits. Methods New Zealand White rabbits were randomly assigned into myocardial infarction( MI) group and MI +( SSYX)( 0. 4g / Kg. d- 1) group after successful establishment of model of MI. Sham operation group were not ligated in the same part of heart. Eight weeks after treatment,rabbits were sacrificed and samples were obtained from peri-infarcted and non-infarcted area. Nerve density were assessed by GAP43 and TH immunohistochemical technique. The nuclear transcription factor( NF-κB),endothelin-l( ET-1),nerve growth factor( NGF),interleukin-1β( IL-1β),tumor necrosis factor- α( TNF-α) and p75-NTR mRNA expression were examined. Results Compared to sham operation group,the densities of immunostaining positive nerve fibers were significantly higher and expressions of NF-κB,ET-1,NGF,IL-1β,TNF-α and p75-NTR mRNA significantly increased in MI group( P 0. 05 for all). Compared to MI group,the densities of GAP43 and TH positive nerve fibers decreased and expression of NF-κB,ET-1,NGF,IL-1β,TNF-α and p75-NTR mRNA were lowered in MI +( SSYX) group( P 0. 05 for all). Conclusion SSYX inhabits neural remodeling,which may be correlated with the inhabition of proinflammatory response and NGF signaling.[Chinese Journal of Cardiac Pacing and Electrophysiology,2014,28( 1) : 59- 62]