Curcumin Induced Adiponectin Expression in Adipocyte Through PPARγ

【Objective】To investigate the potential benefits of curcumin,an extract from traditional medical plants,on adipocyte function.【Methods】Rabbit subcutaneous adipocytes were treated with 5,10,20 μg/mL curcumin for 24 h.One of the major adipokines,adiponectin secretion in supernatant was examined by special ELISA kit.The expression of adiponectin and PPARγ,its upstream regulator,were quantitated by RT-PCR.【Results】①Curcumin increased the adiponectin secretion examined in supernatant in a dose dependent pattern.From 5 μg/mL to 20μg/mL,the secretions of adiponectin were(3.93±0.26),(4.49±0.34),(5.21±0.39) vs(3.13±0.21) μmol/l(control group).②Curcumin induced the expression of PPARγ in cultured adipocyte,and reached its peak value on 20 μg/mL(1.01±0.15) vs(0.48±0.12).The adiponectin mRNA was also increased under treatment of all the doses of curcumin(0.55±0.09),(0.66±0.11),(0.80±0.13) vs(0.40±0.10).【Conclusion】Curcumin is able to induce the expression and secretion of adiponectin in adipocyte,which is related to its upregulation of expression of PPARγ.