Abstract The naturally configurated S‐[(2R)‐2‐acetamido‐3‐(hexadecyloxy)propyl] 2‐(trimethylammonio)ethyl thiophosphate (5), a new amido‐thiophosphate analog of the Platelet Activating Factor (PAF), was obtained in a seven‐step reaction sequence by starting from N‐acetyl‐l‐cysteine (6).