纳米复合材料
材料科学
纳米管
微晶
结晶度
再生(生物学)
骨组织
拉曼光谱
复合材料
骨愈合
化学工程
生物医学工程
碳纳米管
生物
医学
解剖
光学
物理
工程类
细胞生物学
冶金
作者
Suziete B.S. Gusmão,Anupama Ghosh,Alan Silva de Menezes,Antônio Francisco Machado Pereira,Míriam Teresa Paz Lopes,Madaline K. Souza,Dalton Dittz,G. J. P. Abreu,Lucielma Salmito Soares Pinto,Antônio Luíz Martins Maia Filho,G.O.M. Gusmão,Thomas J. Webster,Anderson Oliveira Lobo,Bartolomeu C. Viana
摘要
Strategies for the production of new nanocomposites that promote bone tissue regeneration are important, particularly those that enhance the osteoinduction of hydroxyapatite in situ. Here, we studied and report the synthesis of nanohydroxyapatite and titanate nanotube (nHAp/TiNT) composites formulated at different concentrations (1, 2, 3, and 10 wt % TiNT) by means of a wet aqueous chemical reaction. The addition of TiNT affects the morphology of the nanocomposites, decreasing the average crystallite size from 54 nm (nHAp) to 34 nm (nHAp/TiNT10%), while confirming its interaction with the nanocomposite. The crystallinity index (CI) calculated by Raman spectroscopy and XRD showed that the values decreased according to the increase in TiNT concentration, which confirmed their addition to the structure of the nanocomposite. SEM images showed the presence of TiNTs in the nanocomposite. We further verified the potential cytotoxicity of murine fibroblast cell line L929, revealing that there was no remarkable cell death at any of the concentrations tested. In vivo regenerative activity was performed using oophorectomized animal (rat) models organized into seven groups containing five animals each over two experimental periods (15 and 30 days), with bone regeneration occurring in all groups tested within 30 days; however, the nHAp/TiNT10% group showed statistically greater tissue repair, compared to the untreated control group. Thus, the results of this study demonstrate that the presently formulated nHAp/TiNT nanocomposites are promising for numerous improved bone tissue regeneration applications.
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