翻译(生物学)
合成生物学
编码区
真核翻译
五素未翻译区
计算生物学
折叠(DSP实现)
非翻译区
蛋白质折叠
平动调节
大肠杆菌
生物
翻译效率
重组DNA
蛋白质生物合成
基因
信使核糖核酸
计算机科学
遗传学
细胞生物学
工程类
电气工程
作者
Sang Woo Seo,Jun Yang,Inhae Kim,Joseph Yang,Byung Eun Min,Sanguk Kim,Gyoo Yeol Jung
标识
DOI:10.1016/j.ymben.2012.10.006
摘要
Precise prediction of prokaryotic translation efficiency can provide valuable information for optimizing bacterial host for the production of biochemical compounds or recombinant proteins. However, dynamic changes in mRNA folding throughout translation make it difficult to assess translation efficiency. Here, we systematically determined the universal folding regions that significantly affect the efficiency of translation in Escherichia coli. By assessing the specific regions for mRNA folding, we could construct a predictive design method, UTR Designer, and demonstrate that proper codon optimization around the 5'-proximal coding sequence is necessary to achieve a broad range of expression levels. Finally, we applied our method to control the threshold value of input signals switching on a genetic circuit. This should increase our understanding of the processes underlying gene expression and provide an efficient design principle for optimizing various biological systems, thereby facilitating future efforts in metabolic engineering and synthetic biology.
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