Relationships between binding of benzodiazepines to alpha‐1‐acid glycoprotein and human serum albumin and their retention on the protein and octadecylsilane columns

Correlation between binding parameters of 6 benzodiazepines to free human serum albumin (HSA) and chromatographic retention parameters on the HSA stationary phase (HSA-SP) was better than the similar correlation with alpha-1-acid glycoprotein (AGP). Still better correlation was obtained between binding to both proteins and retention on an octadecylsilane (ODS) column. For 12 benzodiazepines the correlations of the retention parameters on both protein columns with the retention on the ODS column were additionally investigated. In the case of HSA this correlation was good.