生物
遗传学
基因
底漆延伸
起始密码子
核酸序列
胚乳
融合基因
共识序列
融合蛋白
基因组文库
基因组组织
基因组
分子生物学
肽序列
信使核糖核酸
重组DNA
作者
Keqin Chen,Irwin Rubenstein
出处
期刊:Gene
[Elsevier]
日期:1991-11-01
卷期号:107 (2): 205-212
被引量:11
标识
DOI:10.1016/0378-1119(91)90320-b
摘要
We have identified a maize ubiquitin (Ubi) fusion gene (UBF9) by screening a maize W22 genomic phage λ library with a short (16-nucleotide) oligodeoxyribonucleotide probe derived from the sequence for the extension sequence of a yeast UBI3 fusion gene. UBF9 consists of an UB monomer sequence (228 bp long) joined to an extension sequence (237 bp long). The extension sequence encodes a protein of 79 amino acids which shares extensive identity with similar extension aa sequences found in yeast, humans, barley and Arabidopsis thaliana. UBF9 encodes a small-size class of Ubi mRNAs in the maize tissues investigated. The UBF9 transcript is present in high levels in maize endosperm tissues 22 days after pollination. Genomic Southern blots of maize inbred W22 DNA indicate that the fusion gene sequences are present in multiple copies in the maize genome. Primer extension experiments indicate that the transcription start point is located at 80 bp upstream from the translation start codon of UBF9, Two 37-bp tandem repeated A + T-rich sequences are found in the 5'-flanking region of UBF9. The A + T-rich sequences share the motif, AATATTTTATT, which is present in a diverse set of plant genes.
科研通智能强力驱动
Strongly Powered by AbleSci AI