Bizionia fulviae sp. nov., isolated from the gut of an egg cockle, Fulvia mutica

A novel Gram-staining-negative, non-spore-forming, non-flagellated, non-motile, aerobic, saffron-coloured, rod-shaped bacterium that did not produce flexirubin-type pigments was designated strain EM7(T) and was distinct from other members of the genus Bizionia by produce carotenoid-type pigments and being able to grow independently of NaCl. Strain EM7(T) was isolated from the intestinal tract of an egg cockle, Fulvia mutica, which had been collected from the West Sea in Korea. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain EM7(T) belonged to the genus Bizionia, and showed sequence similarity to Bizionia paragorgiae KMM 6029(T) (97.9%) and Bizionia saleffrena HFD(T) (97.73%). Growth occurred on marine agar 2216 at 0-25 °C (optimum, 20 °C) and at pH 6-9 (optimum, pH 7). Growth occurred in the presence of 0-10% (w/v) NaCl (optimum, 2%, w/v, NaCl). The major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C15 : 1 G, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C17 : 0 3-OH and iso-C16 : 0 3-OH. The major respiratory quinone was menaquinone MK-6. The polar lipids of strain EM7(T) comprised phosphatidylethanolamine, three unidentified aminolipids, an unidentified aminophospholipid and two unidentified lipids. The genomic DNA G+C content was 34.8 mol%. Bizionia paragorgiae KMM 6029(T) and Bizionia saleffrena HFD(T) to Bizionia paragorgiae KCTC 12304(T) and Bizionia saleffrena CIP 108534(T), respectively. Thus, it is proposed that the isolate represents a novel species, Bizionia fulviae sp. nov., with strain EM7(T) ( = KACC 18255(T) = JCM 30417(T)) as the type strain.