丝素
神经保护
自愈水凝胶
药理学
体内
化学
医学
材料科学
生物
生物技术
有机化学
丝绸
复合材料
作者
Jiaxin Zhang,Sunao Li,Zhenbei Yang,Chao Liu,Xueshi Chen,Yuqi Zhang,Feng Zhang,Haibin Shi,Xiping Chen,Luyang Tao,Haiyan Shan,Mingyang Zhang
出处
期刊:Biomaterials advances
日期:2022-04-01
卷期号:135: 212743-212743
被引量:8
标识
DOI:10.1016/j.bioadv.2022.212743
摘要
Hydrogen sulfide (H2S), an important endogenous signaling molecule, plays an important neuroprotective role in the central nervous system. However, there is no ideal delivery material or method involving the sustained and controlled release of H2S for clinical application in brain diseases. Silk fibroin (SF)-based hydrogels have become a potentially promising strategy for local, controlled, sustained drug release in the treatment of various disorders. Here, we show a silk fibroin (SF)-based hydrogel with sustained H2S delivery (H2[email protected] hydrogel) is effective in treating brain injury through stereotactic orthotopic injection in a severe intracerebral hemorrhage (ICH) mouse model. In this study, we observed H2[email protected] hydrogel sustained H2S release in vitro and in vivo. The physicochemical properties of H2[email protected] hydrogel were studied using FE-SEM, Raman spectroscopy and Rheological analysis. Treatment with H2[email protected] hydrogel attenuated brain edema, reduced hemorrhage volume and improved the recovery of neurological deficits after severe ICH following stereotactic orthotopic injection. Double immunofluorescent staining also revealed that H2[email protected] hydrogel may reduce cell pyroptosis in the striatum, cortex and hippocampus. However, when using endogenous H2S production inhibitor AOAA, H2[email protected] hydrogel could not suppress ICH-induced cell pyroptosis. Hence, the therapeutic effect of the H2[email protected] hydrogel may be partly the result of the slow-release of H2S and/or the effect of the SF hydrogel on the production of endogenous H2S. Altogether, the results exhibit promising attributes of injectable silk fibroin hydrogel and the utility of H2S-loaded injectable SF hydrogel as an alternative biomaterial toward brain injury treatment for clinical application.
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