Two new alkaloids identified from herb Delphinium trichophorum French, a famous Tibetan drug “Gebu Dilu”

A new benzamide derivative alkaloid (1) and a new hetisine-type diterpenoid alkaloid (2) were isolated from D. trichophorum Franch with fourteen known alkaloids compounds (3∼16, including twelve hetisine-type diterpenoid alkaloids), in which three compounds were discovered firstly from genus Delphinium (10, 15, 16) and three compounds were discovered firstly from species D. ­trichophorum (7, 9, 11). Their structures were elucidated by ­extensive spectroscopic analyses, including 1D NMR, 2D NMR and ESI-MS/HR ESI-MS techniques, along with their anti-inflammatory activities were evaluated by measuring NO production in LPS-stimulated RAW264.7 cells.