Textile dye effluent collected from three different stages like Raw, primary treated and secondary [Outlet] treated effluent in Trippur area and analysed physiochemical parameters using standard methods. These various stages effluent treated to soybean crop to analysed seed germination, shoot length, root length, number leafs, root nodules, number of lateral roots and total chlorophyll content. Raw effluent directly inhibits all the parameters evenly and primary treated effluent also significantly inhibits the soybean crop. Outlet effluent didn’t inhibit the all parameters to compare control. Outlet treated effluent showed the significant result to compare the raw and primary treated effluent.