生物
病毒学
印地语III
牛痘
拉伤
病毒
痘病毒科
限制性酶
DNA
限制性片段
遗传学
重组DNA
基因
解剖
作者
Yongtai Hou,Xiaoxin Yang,Hu Yongqiang
标识
DOI:10.1099/0022-1317-66-8-1819
摘要
HindIII fragments D to P of DNA from a Chinese vaccine strain (Tian Tan) of vaccinia virus have been molecularly cloned into the plasmid pAT153 at the unique HindIII site. The Chinese strain DNA differs from a non-vaccine American strain (WR) in having an additional HindIII fragment (P). Twelve HindIII D clones and 12 HindIII F clones of the Chinese strain were analysed by digestion by EcoRI, BamHI, PstI and XhoI. Two forms of D (designated a and b) and of F (a and b) were demonstrated. In each the differences were detected as the presence of an additional EcoRI site, in Da and Fa. The HindIII Fa and Fb fragments of the Chinese strain were shown to differ significantly from the WR strain in their restriction site maps.
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