固定化酶
咪唑酯
生物传感器
材料科学
基质(水族馆)
微型多孔材料
介孔材料
沸石咪唑盐骨架
电化学
金属有机骨架
组合化学
酶
米氏-门汀动力学
普鲁士蓝
电极
色谱法
化学
无机化学
酶分析
有机化学
纳米技术
吸附
催化作用
地质学
物理化学
复合材料
海洋学
作者
Cheng Zhang,Xuerui Wang,Meng Hou,Xiaoyang Li,Xiaoling Wu,Jun Ge
标识
DOI:10.1021/acsami.7b02803
摘要
The protection effect of metal–organic framework (MOF) provides high stability for immobilized enzyme. The small cavities of MOFs, however, usually result in decreased apparent substrate affinity and enzymatic activity of immobilized enzyme, compared to native enzyme. We synthesized zeolitic imidazolate framework-8 (ZIF-8) with a combination of mesoporous and microporous channels for cytochrome c (Cyt c) immobilization. Compared with native Cyt c, the immobilized Cyt c displayed increased apparent substrate affinity (Michaelis constant Km reduced by ∼50%), ∼128% increased enzymatic activity, and 1.4-fold increased sensitivity in the enzymatic electrochemical detection of H2O2. The immobilized Cyt c-coated screen-printed electrode was applied for the fast detection of residual H2O2 in microliter food samples such as milk and beer, making it promising for the development of efficient biosensors.
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