适体
卡那霉素
检出限
化学
过氧化物酶
线性范围
组合化学
色谱法
生物化学
分子生物学
酶
生物
抗生素
作者
Xuan Zhou,Jiaxin Li,Yimin Hu,Yaohui Wu,Yonghong Wang,Ning Ge
出处
期刊:Analytical Methods
[The Royal Society of Chemistry]
日期:2023-01-01
卷期号:15 (20): 2441-2447
被引量:3
摘要
Kanamycin is used widely in livestock farming due to its antimicrobial properties and low cost, but has led to antibiotic residues in food, which can damage human health. Therefore, there is an urgent need for convenient technology that can be used to detect kanamycin rapidly. We found that Co3O4 nanoparticles (NPs) possessed peroxidase-like activity that catalyzed the oxidation of 3,3',5,5'-tetramethylbenzidine to change color. Interestingly, a target-specific aptamer could regulate the catalytic activity of Co3O4 NPs and inhibit this effect through aptamer-target binding. On the basis of a colorimetric assay combined with an aptamer-regulatory mechanism, the linear range for quantitative detection of kanamycin was 0.1-30 μM, the minimum limit of detection was 44.2 nM, and the total time needed for detection was 55 min. Moreover, this "aptasensor" displayed excellent selectivity and could be applied to detect KAN in milk samples. Our sensor might have promising applications for kanamycin detection in animal husbandry and agricultural products.
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