细胞生物学
细胞周期蛋白依赖激酶1
促成熟因子
生物
减数分裂
卵母细胞
细胞周期蛋白B
细胞周期蛋白B1
卵子发生
激酶
作者
Edgar Del Llano,Rajan Iyyappan,Daria Aleshkina,Tomáš Mašek,Michal Dvoran,Zongliang Jiang,Martin Pospíšek,Michal Kubelka,Andrej Susor
标识
DOI:10.1016/j.ejcb.2022.151210
摘要
In mammalian females, oocytes are stored in the ovary and meiosis is arrested at the diplotene stage of prophase I. When females reach puberty oocytes are selectively recruited in cycles to grow, overcome the meiotic arrest, complete the first meiotic division and become mature (ready for fertilization). At a molecular level, the master regulator of prophase I arrest and meiotic resumption is the maturation-promoting factor (MPF) complex, formed by the active form of cyclin dependent kinase 1 (CDK1) and Cyclin B1. However, we still do not have complete information regarding the factors implicated in MPF activation. In this study we document that out of three mammalian serum-glucocorticoid kinase proteins (SGK1, SGK2, SGK3), mouse oocytes express only SGK1 with a phosphorylated (active) form dominantly localized in the nucleoplasm. Further, suppression of SGK1 activity in oocytes results in decreased CDK1 activation via the phosphatase cell division cycle 25B (CDC25B), consequently delaying or inhibiting nuclear envelope breakdown. Expression of exogenous constitutively active CDK1 can rescue the phenotype induced by SGK1 inhibition. These findings bring new insights into the molecular pathways acting upstream of MPF and a better understanding of meiotic resumption control by presenting a new key player SGK1 in mammalian oocytes. • Out of three mammalian serum-glucocorticoid kinase proteins oocyte expresses only SGK1, with active form dominantly localized in the nucleoplasm. • Suppression of SGK1 activity in the oocyte resulted in the decreased CDK1 activation via CDC25B, negatively influencing nuclear envelope breakdown. • Expression of a constitutively active CDK1 overcame the delay of the nuclear envelope breakdown. • These findings bring new insights into the regulation of MPF via SGK1 in mammalian oocyte.
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