频谱分析仪
微流控
检测点注意事项
吞吐量
化学
重组酶聚合酶扩增
检出限
纳米技术
基因
计算机科学
聚合酶链反应
色谱法
光学
材料科学
物理
生物
电信
生物化学
免疫学
无线
作者
Gaihua Cao,Danqun Huo,Xiaolong Chen,Xianfeng Wang,Shiying Zhou,Shixian Zhao,Xiaogang Luo,Changjun Hou
出处
期刊:Talanta
[Elsevier]
日期:2022-10-01
卷期号:248: 123594-123594
被引量:20
标识
DOI:10.1016/j.talanta.2022.123594
摘要
COVID-19 has erupted and quickly swept across the globe, causing huge losses to human health and wealth. It is of great value to develop a quick, accurate, visual, and high-throughput detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we developed a biosensor based on CRISPR/Cas13a combined with recombinase polymerase amplification (RPA) to detect S and Orf1ab genes of SARS-CoV-2 within 30 min. Most important of all, we developed an automated, portable, and high-throughput fluorescence analyzer (APHF-analyzer) with a 3D-printed microfluidic chip for sensitively detecting SARS-CoV-2, which addressed aerosol contamination issue and provided a more accurate and high-throughput detection during the on-site detection process. The detection limits of S gene and Orf1ab gene were as low as 0.68 fM and 4.16 fM. Furthermore, we used the lateral flow strip to realize visualization and point of care testing (POCT) of SARS-CoV-2. Therefore, profit from the efficient amplification of RPA and the high specificity of CRISPR/Cas13a, APHF-analyzer and the lateral flow strip to simultaneous detection of S gene and Orf1ab gene would be applied as a promising tool in the field of SARS-CoV-2 detection.
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