适体
赭曲霉毒素A
化学
检出限
荧光
核酸外切酶 III
荧光染料
核酸外切酶
色谱法
生物传感器
胶体金
DNA
线性范围
核酸
纳米化学
分析化学(期刊)
真菌毒素
分子生物学
纳米技术
生物化学
纳米颗粒
生物
实时聚合酶链反应
材料科学
食品科学
大肠杆菌
DNA聚合酶
量子力学
有机化学
物理
基因
作者
Renjie Liu,Hua Wu,Lei Lv,Xiaojiao Kang,Chengbi Cui,Jin Feng,Zhijun Guo
标识
DOI:10.1007/s00604-018-2786-6
摘要
This study describes an aptamer based assay for the mycotoxin ochratoxin A (OTA). The method is based on the use of an OTA-specific aptamer, exonuclease (Exo) III, SYBR Gold as a fluorescent probe, and a complementary strand that specifically combines with the aptamer. In the presence of OTA, the aptamer and OTA hybridize, thereby resulting in the formation of ssDNA, which is not digested by Exo III. Intense fluorescence is observed after addition of SYBR Gold (best measured at excitation/emission wavelengths of 495/540 nm). Fluorescence increases linearly with the log of the OTA concentration in the range from 8 to 1000 ng·mL-1. The detection limit is 4.7 ng·mL-1. The assay was applied to the determination of OTA in diluted [2%(v/v)] red wine, and recoveries and RSDs ranged between 93.5% and 113.8%, and between 3.2% and 5.7%, respectively. Graphical abstract In the presence of ochratoxin A (OTA), specific combinations of aptamer and OTA may occur and result in DNA double strands being untied, which avoids being digested by Exo III. Intense fluorescence is observed after SYBR Gold addition.
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