毒液
蝎子
重组DNA
蝎子毒素
化学
生物活性
分子生物学
生物化学
蝎子毒
生物
体外
基因
作者
Sheng-Guo Lin,Xuelin Wang,Xueyao Hu,Yahui Zhao,Ming Zhao,Jinghai Zhang,Yong Cui
出处
期刊:Protein and Peptide Letters
[Bentham Science]
日期:2017-01-27
卷期号:24 (3): 235-240
被引量:10
标识
DOI:10.2174/0929866524666170117142404
摘要
Scorpion venom contains a large variety of biologically active peptides. However, most of these peptides have not been identified and characterized. Peptides with three disulfide bridges, existing in the scorpion venom, have not been studied in detail and have been poorly characterized until now. Here, we report the recombinant expression and functional characterization of two kinds of venom peptides (BmKBTx and BmNaL-3SS2) with three disulfide bridges. This study adopted an effective Escherichia coli system. The genes for BmKBTx and BmNaL-3SS2 were obtained by polymerase chain reaction and cloned to the pSYPU-1b vector. After expression and purification, the two recombinant proteins were subjected to an analgesic activity assay in mice and whole-cell patchclamp recording of hNav1.7-CHO cell lines. Functional tests showed that BmKBTx and BmNaL- 3SS2 have analgesic activity in mice and can interact with the hNav1.7 subtype of the voltage-gated sodium channel (VGSC). Scorpion venom is rich in bioactive proteins, but most of their functions are unknown to us. This study has increased our knowledge of these novel disulfide-bridged peptides (DBPs) and their biological activities. Keywords: BmKBTx, BmNaL-3SS2, recombinant expression, analgesic activity, electrophysiology, hNav1.7, disulfide bridge.
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