Preparation of Chitooligosaccharides with Degree of Polymerization of 6 to 8

Objective To study the preparation technology of chitooligosaccharides with degree of polymerization of 6～8.Methods Chitosan was hydrolyzed by special enzymes,then the hydrolysis product was successively ultrafiltered,dried and examined with TLC,HPLC and MALDI-TOF-MS.Results The product was white powder and mainly contains chitooligosaccharides with degree of polymerization of 6～8.Conclusion This method can be used for the preparation of chitooligosaccharides with degree of polymerization of 6～8.