Study on antioxidant activity of Sarcopyamis nepalensis Wall extracts

Effectively active components of Sarcopyamis nepalensis Wall were extracted using pure water,60% ethanol and anhydrous ethanol respectively,and then total flavonoids content and the antioxidant potency of the three kinds of extracts were investigated,employing various established in vitro systems including total antioxidative capacity,superoxide and hydroxyl radical inhibiting ability,reducing power. According to the results,total flavonoid content of pure water,60% ethanol and anhydrous ethanol three kinds of solvent extracts were 0.081,0.311 and 0.217mg/mL. Three kinds of extracts had good antioxidant activity;among them 60% ethanol extract was the strongest vitality in total antioxidant potency,hydroxyl radicals and superoxide radical inhibiting ability. Antioxidant ability of three kinds of extracts showed dose-effect relationship within 1～7g/L,moreover,there was compatible corresponding relation between antioxidant ability and the test results of reduction ability.