The experiment aimed to study the effect of diet supplementation with locally prepared probiotic ( Iraqi probiotic ), probiotic (Aspergillus.niger) and their mixture on some productive traits of acclimated Hy-Line layers . A total of 120 Hy-Line hens , 44 weeks old , were randomly allocated into four treatment groups with three replicates for each treatment . The treatment groups were: the control ( T1) which fed on common diet. Laying Hens in T2 and T3 were fed a standard diets supplemented with localy prepared probiotic in treatment 2 (T2) and probiotic (A.niger) in treatment 3 (T3) at a rate of 5g / kg die for each . Hens in treatment 4 group (T4) were fed with diet supplemented with mixture of two types of probiotic which contained an equal quantity of local prepared probiotic and probiotic(A.niger) , i.e. 2.5 g local prepared probiotic and 2.5 gm of probiotic (A.niger) . Results showed that the dietary supplementation with localy prepared probiotic , probiotic (A.niger) and their mixture resulted in a significant improvement in hen day production and egg weight . There were no significant differences between the treatment groups and control as regards livability percentage .