Determination of ursolic acid and oleanolic acid in Salvia chinensis Benth by HPLC

Objective To establish a method for determination of ursolie acid and oleanolic acid contents in Salvia chinensis Benth.Methods HPLC-DAD was used;the column was Agilent HC18(4.6 mm×250 mm,5μm);mobile phase was A:0.1%(v/v)formic acid solution,B:methanol,A∶B = 14∶86.The flow rate was 1.0 ml/min;the temperature of column was 20 ℃;the detection wavelength was set at 205 nm;the injection volume was 50 μl;the running time was 35 min.Results Ursolic acid and oleanolic acid were separated in baseline,the linear range was 11.55~462.0 μg/ml for ursolic acid and 5.265~210.6 μg/ml for oleanolic acid.The result of intra-day and inter-day precisions were both within 5%(n=3),and the average recovery was 100.47%(RSD= 1.95%,n=6)and 101.88%(RSD= 2.98%,n=6).Conclusion The method was rapid,convenient,accurate,and suitable for quality control of ursolic and oleanolic acid in Salvia chinensis Benth.