Determination of Diniconazole Residue in Fruits by HPLC-MS/MS

An high performance liquid chromatography-tandem mass spectrometry(LC-MS/MS) analytical method for the determination of diniconazole residues in the fruits has been developed.Samples are liquid-liquid extracted with acetone-hexane and clean up with dispersive solid phase extraction(DSPE).The analyte is separated on an ODS-C18 column with acetonitrile-0.1% formic acid solution as the mobile phase.The target compound is detected by LC-MS/MS(ESI +) under the multiple reaction monitoring(MRM) mode using an external standard method.The linear relationship of this method was good between 0.002~0.2 mg/L concentration range.The limit of quantification(LOQ) was 0.001 mg/kg.The recoveries of diniconazole ranged from 78.8% to 108.0% at the three spiked levels of 0.005~0.1 mg/kg and the relative standard deviations(RSDs) were in range of 2.1%~6.9%.