Effect of exogenous transforming growth factor-beta 2(TGF-β_2) and bone morphogenetic protein 2(BMP_2) and allografts bone on radius defects healing of experimental rabbits

[Objective]To investigate the effect of exogenous TGF-β2and BMP2 and allografts bone on radius defects.[Method]The model of adult rabbit radius defects was made and treated with TGF-β2 and BMP2 respectively or in combination and allografts bone in the radius defects.A group:BMP2 and allografts bone.B group:autogenous bone.C group:TGF-β2 and allografts bone.D group:BMP2 and TGF-β2 and allografts bone.Fracture healing was evaluated in different time by radiograph,biomechanical tests,measurement of the bone mineral density and calcium content in callus.[Result]The results of B group excelledthose of A,C,D group in aspects of calcium content in callus,the ability of radius defects and biomechanical strength of bone(P0.01).The effect of C group was better than that of A group(P0.01),while D group brought better result than A and C group(P0.05).[Conclusion]TGFβ2 and BMP2 play an important role on radius defects healing.Fracture healing and callus content and mechanics intensity can be conspicuous promoted by local application of exlgenous TGF-β2 and BMP2,meanwhile,the healing time with fracture healing can get close the healing time with autogenous utmostly,decurtating the healing time of bone defect of patient who need long segment graft bone would be shortende the effect of single usage of TGF-β2 is better than that of BMP2.The result will be further enhanced if they are applied together with synergistic effect.At the same time,it could provid dependable index for determiner metrology and the agent that should be combined with allograft bone.