二乙酰氧基癸酚
化学
赤眼蜂
色谱法
单克隆抗体
真菌毒素
检出限
免疫分析
抗体
食品科学
生物
免疫学
作者
Xiaoqian Tang,Jing Wu,Wenqin Wu,Zhaowei Zhang,Weiqi Zhang,Qi Zhang,Wen Zhang,Xiaomei Chen,Peiwu Li
标识
DOI:10.1021/acs.analchem.9b03933
摘要
Diacetoxyscirpenol (DAS) is a type A trichothecene mycotoxin with low molecular weight, and with respect to its toxicity and the occurrence in food and feed, it is known as a potential risk for public and animal health. In the present study, first, a sensitive and specific monoclonal antibody (5E7) was developed. Then, the antibody was applied to develop a competitive-type pressure-dependent immunosensor (CTPDI). The Au@PtNP was synthesized and labeled with goat antimouse antibody (Au@PtNPs-IgG). Finally, the concentration of DAS was negatively correlated with the pressure signal. In the presence of optimal conditions, matrix-matched calibration curves were plotted for wheat samples, in which an optimal IC50 value (half maximal inhibitory concentration) of 3.08 ng/g was achieved. The CTPDI was further applied to detect natural and blind wheat samples, and validation was carried out by liquid chromatography-tandem mass spectrometry. The results showed that CTPDI was highly appropriate and accurate for detection of DAS in wheat.
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