Evaluation of different direct and indirect SELEX monitoring methods and implementation of melt-curve analysis for rapid discrimination of variant aptamer sequences
Rezvan Mohammadinezhad,Seyed Amir Hossein Jalali,Hamid Farahmand
出处
期刊：Analytical Methods [Royal Society of Chemistry] 日期：2020-01-01卷期号：12 (30): 3823-3835被引量：14
标识
DOI：10.1039/d0ay00491j
摘要
EMSA is the most cost-beneficial and accurate method for screening SELEX for protein targets and melt curve analysis in colony real-time PCR is a robust and effective method for distingsuishing variant aptamer sequences.
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