重组DNA
金黄色葡萄球菌
CpG寡核苷酸
生物
微生物学
毒素
病毒学
免疫
抗体
免疫学
细菌
基因
生物化学
基因表达
遗传学
DNA甲基化
作者
Cecilia María Camussone,Ivana Gabriela Reidel,Ana Molineri,J. Cicotello,Claudia Carabajal Miotti,Guillermo Alejandro Suarez Archilla,Cesarina Curti,Carolina Veaute,Luis Fernando Calvinho
标识
DOI:10.1016/j.rvsc.2022.02.014
摘要
The aims of the present study were to evaluate the ability of a subunit vaccine composed of recombinant molecules of α-toxin, β-toxin, FnBPA and ClfA, formulated with cationic liposomes and CpG-ODN, to confer protection against natural S. aureus intramammary infection (IMI) and to assess the antibody response against the vaccine components. A stringent criterion based on molecular identification of the isolates was used to define IMI. The proportion of animals that developed new S. aureus IMI was higher in the Control group compared with the Vaccine group (reduction of 60.7%), and time to new S. aureus IMI was higher for animals in the Vaccine group compared with animals in the Control group, although not statistically significant. Molecular identification of the isolates allowed the detection of S. aureus pulsotypes that appeared transiently in milk and others that were able to establish IMI, providing a new perspective to define parameters related to the definition of new IMI and cures. Specific IgG, IgG1 and IgG2 levels against the four recombinant proteins included in the vaccine were significantly increased in the vaccinated group and the recombinant α-toxin included in the vaccine generated antibodies that reduced significantly the haemolytic activity of native α-toxin. Data reported in the present study indicate a possible effect on both the proportion of animals developing new IMI and the time to new S. aureus IMI, but the incidence of disease within the study was too low to provide statistical confirmation.
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