Vibrio sinus sp. nov., a marine bacterium isolated from coastal seawater

A Gram–straining–negative, facultatively anaerobic, motile by means of a polar flagellum and rod-shaped marine bacterium, designated S4M6 T , was isolated from surface seawater collected in Dongshan Bay (Fujian, PR China). Phylogenetic analysis based on 16S rRNA genes, phylogenomic analysis of single-copy gene families and whole genome data indicated that S4M6 T represented a member of the genus Vibrio . The closest phylogenetic relatives of S4M6 T were Vibrio marisflavi CGMCC 1.8994 T (97.8 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis LMG 25438 T (96.9 %), Vibrio gangliei SZDIS-1 T (96.2 %) and Vibrio aestivus M22 T (96.1 %). The growth of S4M6 T occurred at 15–35 °C (optimum 28 °C), pH 4.0–9.0 (optimum 5.0–7.0) and in the presence of 2–5 % (w/v) NaCl (optimum 3 %). The predominant fatty acids (>10 %) are C 16 : 0 , summed feature 3 (C 16 : 1 ω7 c and/or C 16 : 1 ω6c) and summed feature 8 (C 18 : 1 ω7 c and/or C 18 : 1 ω6 c ). The DNA G+C content of the assembled genomic sequences was 43.4 % for S4M6 T . Average nucleotide identity (ANI) values between S4M6 T and the reference species were lower than the threshold for species delineation (95–96 %); in silico DNA–DNA hybridization further indicated that S4M6 T had less than 70 % similarity to its relatives. On the basis of the polyphasic evidence, strain S4M6 T is proposed to represent a novel species of the genus Vibrio , for which the name Vibrio sinus sp. nov. is proposed. The type strain is S4M6 T (= KCTC 92312 T = MCCC 1K06167 T ).