Wetting of Cell Aggregates on Microdisk Topography Structures Achieved by Maskless Optical Projection Lithography

Abstract Cell aggregates as a 3D culture model can effectively mimic the physiological processes such as embryonic development, immune response, and tissue renewal in vivo. Researches show that the topography of biomaterials plays an important role in regulating cell proliferation, adhesion, and differentiation. It is of great significance to understand how cell aggregates respond to surface topography. Herein, microdisk array structures with the optimized size are used to investigate the wetting of cell aggregates. Cell aggregates exhibit complete wetting with distinct wetting velocities on the microdisk array structures of different diameters. The wetting velocity of cell aggregates reaches a maximum of 293 µm h −1 on microdisk structures with a diameter of 2 µm and is a minimum of 247 µm h −1 on microdisk structures of 20 µm diameter, which suggests that the cell‐substrates adhesion energy on the latter is smaller. Actin stress fibers, focal adhesions (FAs), and cell morphology are analyzed to reveal the mechanisms of variation of wetting velocity. Furthermore, it is demonstrated that cell aggregates adopt climb and detour wetting modes on small and large‐sized microdisk structures, respectively. This work reveals the response of cell aggregates to micro‐scale topography, providing guidance for better understanding of tissue infiltration.