Detection of BRCA1,CDH1,DKK1 and SFRP1 methylation in peripheral blood cells of patients with Metastatic breast cancer

Objective To investigate the relationship of BRCA1,CDH1,DKK1 and SFRP1 gene methylation with ER status,relapse and metastasis in patients of Metastatic breast cancer.Methods Explore the methylation status of four genes BRCA1,CDH1,DKK1 and SFRP1 in 115 metastasis breast cancer patients' peripheral blood.65 patients with healthy controls with comparison.Results Percent of BRCA1.CDH1 and SFRP1methylation in patients are significantly higher compared to healthy controls.About 27.5% of patients with ER positive tumor were CDH1 methylated in peripheral blood cells,while 47.8% with ER negative tumor were methylated.In contrast,ER positive patients had a higher rate of SFRP1 methylation compared to ER negative patients.CDH1 methylation was negatively related to distant metastasis.Conclusion The four candidate genes methylation in PBC were strongly associated with clinical progress for breast cancer patients.CDH1 and SFRP1 methylation were significantly related with ER status.They provide certain reference for disease assessment,treatment and prognostic analysis.