聚苯胺
材料科学
生物传感器
适体
检出限
生物污染
纳米技术
化学工程
聚合物
化学
生物化学
色谱法
膜
生物
工程类
复合材料
聚合
遗传学
作者
Xiaoyan He,Nan Sun,Hui Jia,Miaomiao Hou,Zheping Tan,Xiaoquan Lu
标识
DOI:10.1021/acsami.2c10081
摘要
As an energy supplier, ATP plays an important role in various life activities, and there is an urgent need to develop an effective means of detecting ATP. However, the traditional sensors face serious nonspecific adsorption. In this work, an antifouling electrochemical biosensor based on the interpenetrating network of Y-DNA scaffold and polyaniline hydrogel was designed for ATP detection. The polyaniline hydrogel was conducive to the transport of electrons and ions, the structure of Y-DNA cross-linked by ATP aptamers in the polyaniline hydrogel achieved the effect of signal amplification. Super hydrophilic cellulose nanocrystals (CNCs) and zwitterion polypeptide sequence (Pep) were doped to play a synergistic antifouling effect. The hydrogel sensor we have built has a wide linear range of 0.1 pM-1 μM for ATP detection and a low detection limit of 0.025 pM (S/N = 3). For ATP detection in actual serum samples, the recovery of this sensor was 99.5%-106%, and the relative standard deviation was 0.4%-2.88%. It is proven that the sensor has good ATP detection performance, and it will provide a certain reference value for the detection of other biological small molecules.
科研通智能强力驱动
Strongly Powered by AbleSci AI