化学
乙酰胆碱酯酶
基质(水族馆)
罗丹明B
色谱法
吸光度
重复性
拉曼散射
拉曼光谱
酶
生物化学
催化作用
光催化
地质学
物理
光学
海洋学
作者
Tianao Li,Tao Sui,Binyu Wang,Ke Xu,Sheng Zhang,Xiaojian Cao,Yi Wang,Weiping Qian,Jian Dong
出处
期刊:Talanta
[Elsevier]
日期:2023-01-01
卷期号:252: 123867-123867
标识
DOI:10.1016/j.talanta.2022.123867
摘要
Accurate discriminating nerve fibers is a prerequisite for right suturing nerves in nerve transfer operation. Various methods have been developed for identification of motor and sensory fibers, but no simple method meets the requirements in clinic. In this study, a surface-enhanced Raman scattering (SERS) lever strategy is designed and developed to detect Acetylcholinesterase (AchE) ultrasensitively, in which using produced thiocholine with weak intrinsic Raman activity (four ounces) to adjust absorbance of Rhodamine B with strong intrinsic Raman activity (thousand catties) on SERS-active substrates is to increase the sensitivity. Employing a miniaturized SERS substrate, SERS-active microneedles, is to decrease the volume of enzymolysis systems. Adopting an internal reference is to increase the repeatability of collected signal. The ultrasensitive AchE detection method discriminate samples with four times of difference in enzyme activity between 1-1 × 10-4 U/mL in about 10 min of enzymolysis time. AchE amounts in 2-mm-long segments of ventral and dorsal roots were about 0.00025-0.001 U and 0.01-0.02 U, respectively. The developed method would be a reliable method met the requirements of identifying motor and sensory fibers in clinic.
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