B细胞激活因子
受体
癌症研究
B细胞
慢性淋巴细胞白血病
免疫学
生物
细胞生物学
化学
白血病
抗体
生物化学
作者
Derek P. Wong,Nand Kishor Roy,Keman Zhang,Anusha Anukanth,Abhishek Asthana,Nicole J. Shirkey-Son,Samantha K. Dunmire,Bonnie Jones,Walker S. Lahr,Beau R. Webber,Branden S. Moriarity,Paolo Caimi,Reshmi Parameswaran
标识
DOI:10.1038/s41467-021-27853-w
摘要
B cell-activating factor (BAFF) binds the three receptors BAFF-R, BCMA, and TACI, predominantly expressed on mature B cells. Almost all B cell cancers are reported to express at least one of these receptors. Here we develop a BAFF ligand-based chimeric antigen receptor (CAR) and generate BAFF CAR-T cells using a non-viral gene delivery method. We show that BAFF CAR-T cells bind specifically to each of the three BAFF receptors and are effective at killing multiple B cell cancers, including mantle cell lymphoma (MCL), multiple myeloma (MM), and acute lymphoblastic leukemia (ALL), in vitro and in vivo using different xenograft models. Co-culture of BAFF CAR-T cells with these tumor cells results in induction of activation marker CD69, degranulation marker CD107a, and multiple proinflammatory cytokines. In summary, we report a ligand-based BAFF CAR-T capable of binding three different receptors, minimizing the potential for antigen escape in the treatment of B cell cancers.
科研通智能强力驱动
Strongly Powered by AbleSci AI