黄芩素
化学
内化
谷胱甘肽
内吞作用
细胞生物学
炎症
介孔二氧化硅
细胞
纳米技术
生物物理学
材料科学
生物化学
介孔材料
药理学
免疫学
医学
生物
催化作用
酶
作者
Xuan Li,Chuan Wang,Leilei Wang,Regina Huang,Wai-Chung Li,Xinna Wang,Sarah Sze Wah Wong,Zongwei Cai,Ken Cham‐Fai Leung,Lijian Jin
标识
DOI:10.1016/j.jcis.2022.01.091
摘要
Precise modulation of immuno-inflammatory response is crucial to control periodontal diseases and related systemic comorbidities. The present nanosystem with the controlled-release and cell-penetrating manner enhances the inflammation modulation effects of baicalein in human gingival epithelial cells (hGECs) for better oral healthcare.We constructed a red-emissive mesoporous silica nanoparticle-based nanosystem with cell-penetrating poly(disulfide) (CPD) capping, through a facile in-situ polymerization approach. It was featured with a glutathione-responsive manner and instant cellular internalization capacity for precisely delivering baicalein intracellularly. Laboratory experiments assessed whether and how the nanosystem per se with the delivered baicalein could modulate immuno-inflammatory responses in hGECs.The in-situ polymerized CPD layer capped the nanoparticles and yet controlled the release of baicalein in a glutathione-responsive manner. The CPD coating could facilitate cellular internalization of the nanosystem via endocytosis and thiol-mediated approaches. Notably, the intracellularly released baicalein effectively downregulated the expression of pro-inflammatory cytokines through inhibiting the NF-κB signaling pathway. The nanosystem per se could modulate immuno-inflammatory responses by passivating the cellular response to interlukin-1β. This study highlights that the as-synthesized nanosystem may serve as a novel multi-functional vehicle to modulate innate host response via targeting the NF-κB pathway for precision healthcare.
科研通智能强力驱动
Strongly Powered by AbleSci AI