拟肽
肽
肽库
组合化学
化学
有孔小珠
蛋白酵素
高通量筛选
生物化学
小分子
计算生物学
肽序列
生物
酶
复合材料
基因
材料科学
作者
Fernanda Bononi,Leonard G. Luyt
出处
期刊:Methods in molecular biology
日期:2015-01-01
卷期号:: 223-237
被引量:8
标识
DOI:10.1007/978-1-4939-2020-4_15
摘要
Combinatorial one-bead-one-compound (OBOC) peptide library screening has proven to be a powerful tool for identification of small molecules, peptides, or peptidomimetics against a variety of specific targets such as cell surface receptors, protein kinases, proteases, and phosphatases. With each bead displaying many copies of a single chemical entity, millions of compounds can be rapidly synthesized and screened with whole-cell binding on-bead functional assays. Here we describe the methodology for the synthesis, screening, and sequence deconvolution of an OBOC peptide library analyzed for affinity to a cancer cell line.
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