纳米器件
原位
信号转导
DNA损伤
细胞生物学
纳米技术
细胞信号
DNA
材料科学
生物物理学
计算生物学
化学
生物
生物化学
有机化学
作者
Hai Shi,Yanxia Wang,Jing Zheng,Limin Ning,Yue Huang,Anzhi Sheng,Tianshu Chen,Yang Xiang,Xiaoli Zhu,Genxi Li
出处
期刊:ACS Nano
[American Chemical Society]
日期:2019-10-11
卷期号:13 (11): 12840-12850
被引量:27
标识
DOI:10.1021/acsnano.9b05082
摘要
Fluorescence imaging tools enable the in situ visualization of those molecules involved in various cell signaling pathways, but directly describing these pathways instead of the separate mediators in situ is much more meaningful and still full of challenges. In this work, a dual-responsive DNA nanodevice that allows the available imaging of an apoptotic signaling pathway in living cells has been developed. The nanodevice is constructed through assembling an elaborately designed Y-shaped DNA (Y-DNA) layer on gold nanoparticles (AuNPs). Only if an apoptotic signaling pathway involving the manganese superoxide dismutase (MnSOD) mRNA and downstream cytochrome c (Cyt c) is presented to serve as the input, the nanodevice can perform an "AND" logic gate operation, disassembling the Y-DNA from the AuNP surface and thereafter outputting a fluorescence signal. In comparison with the fluorescence imaging methods that target individual specific molecules, our strategy allows direct profiling of a specific signaling pathway by connected characterization of two correlative targets. The programmable feature of this strategy also shows the potential for the profiling of other signaling pathways. The concept of studying the line connecting two points will contribute to the systematic interrogation on the signaling networks in situ as the networks are composed of lines instead of individual points.
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