纤维素酶
鲍曼不动杆菌
纤维素
不动杆菌
蚯蚓粪
酶
细菌
分离(微生物学)
食品科学
生物
微生物学
化学
生物化学
抗生素
生态学
营养物
铜绿假单胞菌
遗传学
作者
A. Karthika,R. Seenivasagan,Ramesh Kasimani,Olubukola Oluranti Babalola,Vasanthy Muthunarayanan
标识
DOI:10.1016/j.wasman.2020.06.036
摘要
In the current scenario, used paper cups are disposed of without any proper treatment, thereby damaging the environment. Hence, the vermicomposting technique is preferred for managing these wastes. The ability of bacterial strains on cellulase enzyme (Endoglucanase, exoglucanase and β-glucosidase) production at altered pH and temperatures were focused in this study. Among nine bacterial strains Acinetobacter baumannii was found to have high enzyme activity. HPLC analysis confirms that about 45% of cellulose degradation occurred due to the action of bacterial consortia at 37 °C with pH 7. The overall period required for degradation takes only three months with the help of bacterial consortia while comparing to our previous study, which takes six months. The insilico study on three cellulose-degrading enzymes sequence were retrieved from NCBI, and analysed for multiple sequence alignment and phylogenetic tree construction. From the analysis, the endoglucanase SVK46152 (Acinetobacter baumannii) sequence got docked with cellopentaose with a high score value -11.07. Thereby we confirm that organism Acinetobacter baumannii was effective in paper cup degradation.
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